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Quantitative polymerase chain reaction (PCR) was performed in duplicates using TaqMan gene expression assays on a CFX96 Touch Real-Time PCR machine (Bio-Rad, Tokyo, Japan)

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3 Results 3.1 Vitamin B12 measurements From January 2016 to December 2023, a total of 84 million vitamin B12 measurements were obtained from both public and private healthcare systems in Brazil (Figure 1A)